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They cleared them, except for small deletions.
However, screening for small deletions of FMR1 may be of clinically utility.
These included scripts to look for small deletions, but aside from eat-5 (ad1402 ) we found none in these mutants.
Genomic DNA samples were screened for small deletions, insertions, and point mutations using the Mitoscreen Assay System (Transgenomic Ltd ,Glasgow, UK).
Thus, the screening for small deletions is carried out in a separate execution of the algorithm where a deletion is treated as a 'fifth base'.
For small deletions, Haplotype Caller performed the best when compared to both Unified Genotyper (McKenna et al., 2010) and FreeBayes (Garrison and Marth, 2012), especially for larger deletions.
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Thus, double-strand break pathways may be particularly relevant for small deletion formation and are therefore candidate mechanisms for salamanders' slow DNA loss.
Flies heterozygous for small deletion knockout alleles of the Malic enzyme gene (Men ) have greater than expected levels of malic enzyme (MEN) protein activity that are not simply physiological up-regulation (Merritt et al. 2005; 2009; Lum and Merritt 2011; Figure 1).
Therefore, it is not suited for smaller deletions or duplications.
This is especially true for smaller deletions of just a few nucleotides that can remain undetected.
After increasing the frequency of the deletion in the sample from 0.2%to1%1%, the power of the DELISHUS algorithm increases significantly and notably for smaller deletions (Fig. 7).
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