Your English writing platform
Discover LudwigExact(3)
For slice preparations animals were deeply anaesthetized with isoflurane and then killed by decapitation.
The critical constraint in this more sophisticated approach to tracer injection is the requirement for optical access, making it practical for slice preparations, retinal flat mounts, or the more superficial layers of the cerebral cortex, but impractical for deeper brain regions.
We have carried out these experiments using the standard approach for slice preparations, as described by Studer et al., although we used 6 week old animals, and not 'pups' (Studer et al., 2014).
Similar(57)
Mice were anesthetized (Ketamine 150 mg/kg/Xylazine 10 mg/kg) and transcardially perfused with aCSF for slice preparation on days 11–14.
Control birds were removed from the communal room and immediately sacrificed for slice preparation.
ACSF for slice preparation was of the following composition (in mM): 124 NaCl, 3 KCl, 1.25 KH2PO4, 5 MgSO4, 3.4 CaCl2, 10 D-glucose, 26 NaHCO3.
Mice (2∼3 months old) housed under standard condition were processed for slice preparation and electrophysiological experiments were performed as previously described [49].
Escherichia coli strains used for SLiCE preparation.
The techniques for slice preparation have been described previously (Gorji and Speckmann, 2004).
Adult mice housed under standard conditions were anaesthetized 19 21 days after retroviral injection and processed for slice preparation as previously described (Ge et al., 2006).
For slice preparation, 5 to 8-day-old (P5 P8) animals were decapitated, the brains were removed and placed into ice-cold cutting solution containing the following (in mM): 252 sucrose, 2.5 KCl, 1.25 NaH2PO4, 5 MgCl2, 0.5 CaCl26 26 NandO3, and 10 glucose.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com