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To determine the stability of the transformed DNA, liquid cultures of RQ7/pDH10 and Tm/pDH10 were transferred every 12 h, for six consecutive times, to fresh media in the presence or absence of kanamycin.
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Section 4 suggests using a deterministic backoff for two consecutive times after a successful transmission.
The LD cycle was then advanced (n=5) or delayed (n=4) for six hours every four days for four consecutive times.
Our proposal is that a station chooses a deterministic backoff for two consecutive times after a successful transmission.
We call the protocol that uses a deterministic backoff for two consecutive times after each successful transmission CSMA/E2CA.
(iii) We suggest the use of a deterministic backoff for two consecutive times after a successful transmission.
Specifically, we advocate for the use of a deterministic backoff for two consecutive times after each successful transmission, and we name this improved protocol CSMA/E2CA.
Throughout the paper, we have considered the CSMA/E2CA protocol that uses a deterministic backoff for two consecutive times after each successful transmission.
A simple approach to avoid the aforementioned unwished behaviour consists in preventing that the same message moves for two consecutive times in a clockwise direction or two consecutive times in a counter-clockwise direction.
This procedure was repeated for three consecutive times.
For the measurement of tissue bacterial outgrowth, aliquots of 0.1 ml of the homogenates were diluted 1 10 into sterile 0.9% NaCl for four consecutive times.
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