Sentence examples for for single staining from inspiring English sources

Briefly for single staining, synoviolin expression was measured by immunohistochemistry using 12.5 µg/ml of a polyclonal rabbit antibody against synoviolin (Abgent).

For single staining of Bub3, Myc6-Bub3, and α-tubulin, oocytes were fixed in 4% paraformaldehyde in PBS (pH 7.4) for at least 30 minutes at room temperature.

Counting was performed in 16 um sections for single staining, and in whole mount with isolated blood cells for double staining.

3-amino-9-ethyl-carbazole (red color) was used as the substrate followed by counterstaining with hematoxylin for single staining according to previously described protocols [5] [7].

For single staining with Wnt3a, sections were incubated in rabbit anti Wnt3a (Abcam, Science Park, Cambridge UK; 5 ug/ml in 0.5% Triton with 1% goat serum) overnight in a humidified chamber at room temperature.

For single staining, the cells were fixed using 4% paraformaldehyde according to the standard protocol, blocked with blocking/permeating buffer (PBS with 10% goat serum and 0.3% Triton-X100) and then incubated with primary antibodies for 1 hour at 37°C.

The IDEAS compensation wizard was used to create a compensation matrix for single stained image files.

The reagents used for the staining were Polymer HRP Detection System PV-90022, ZSGB-BIO) for single-staining and DouSP KIT-99999, MXB) for double-staining.

Anti-mouse and/or anti-rat compensation beads (BD 552843 and 552845, respectively) were used for single-stain antibody controls.

Anti-mouse or anti-rat compensation beads (BD 552843 and 552845, respectively) or both were used for single-stain antibody controls.

Before we adopted the method of dual staining of FoxP3 and the active form of caspase 3, we confirmed that the results of single staining for both proteins were similar to results generated by their simultaneous staining data not shown.