This induction seems to be particularly important at the two pH values tested during the early (2h) timepoint we observed for several transcripts (Figure 5).
Interestingly, for several transcript-protein pairs the expression correlated when oxygen availability was high, but varied in low oxygen conditions, especially when only 0.5% oxygen was provided (members of groups 2, 4 and 5).
Examining changes in transcript abundance that occurred with the phenotypic EMT consistent differences were observed for several transcripts that contribute to the canonical mesenchymal phenotype.
We were able to assess the degree of successful subtraction for several transcripts by performing regular PCR on subtracted and unsubtracted poly-A cDNA pools.
For several transcripts, a reliable prediction of general biochemical activity can be made, but predictions neither suggest nor challenge a role in paclitaxel synthesis.
We performed real-time PCR to validate changes in gene expression for several transcripts altered by miR-9 overexpression in BMMCs.
In addition to the sleep-related genes that appeared in the top-ranked fold-change lists, we also found significant differential expression for several transcripts of the brain-specific marker for sleep homeostasis Homer1.
This was the case for several transcripts whose products are involved either in the biosynthesis of JA or in JA-mediated signalling, defence-related genes, transcription factors and redox homeostasis.
We performed real-time PCR to validate changes in gene expression for several transcripts altered by miR-9 overexpression, including mast cell chymase (CMA1), interferon-induced transmembrane protein 3 (IFITM3), and PDZK1 interacting protein 1 (PDZK1IP1).
The rnr1-3 mutaccumulatesates precursors for several transcripts encoded in the chloroplast ribosomal operon, but is particularly deficient in the accumulation of both precursor and mature forms of 5S rRNA, which is encoded by the rrn5 gene that lies just upstream of trnR, which encodes tRNAArg.
