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Sequencing was performed on an ABI 3730 DNA sequencer using BigDye V3.1 (Applied Biosystems) and M13F primer were used for sequencing from the 5' end of cDNAs.
We obtained 20 clones for sequencing from 2 3 independent PCR reactions for each subject.
Several approaches have been developed to select the regions of interest for sequencing from the genome [20].
Briefly, the whole-genome shotgun sequencing method and the 3-Kb, 8-Kb, and 40-Kb DNA libraries were used for sequencing from both sides of library inserts.
Here, we present a method where PCR products are used to capture targets for sequencing from pooled sequencing libraries of multiple individuals, using standard laboratory equipment.
We selected 'extreme' accessions for sequencing from based on WC-1 NpolyQ length; we defined extreme as being on one of the tails of the NpolyQ length distribution.
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In order to simplify this analysis, we have developed SmashCell (Simple Metagenomics Analysis SHell-for sequences from single Cells).
The Illumina and 454-sequencing reads were mapped against these reduced unigene sets with bowtie2 [ 55] and bwa for sequences from the 454-sequencing [ 56].
Sample shadow detection results for sequences from CDnet are shown in Fig. 6.
Rarefaction analyses showed similar diversity patterns for sequences from isolates and molecular approaches, except for Alphaproteobacteria where cultivation retrieved a higher diversity per unit effort.
Panning the library against two carbohydrate-specific antibodies yielded anti-idiotypic dAbs and enriched solely for sequences from the VH subpopulation of the library.
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