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GenElute™ PCR clean-up column (Thermo Fisher Scientific, Germany) was used for purification before sending the extracted DNA for sequencing, according to the manufacturer's instructions.
After preparation of the library, an Illumina HiSeq 2000 platform was used for sequencing according to the manufacturer's instructions and paired-end reads were obtained.
The isolates used to represent sequence types from other regions then Australia were selected for sequencing according to previous studies [16], [29], [30].
After PCR amplification, emulsions were broken using butanol, the beads were washed, enriched, and terminal transferased before quantification and deposition onto a slide for sequencing according to manufacturer's instructions.
Libraries were prepared for sequencing according to the manufacturer's instructions.
Positive samples by SYBR® Green were selected for sequencing according to their (melting temperature) Tm.
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The primers used for PCR amplification were designed for amplicon sequencing according to Illumina/Solexa guidelines.
Primers used for PCR amplification were designed for amplicon sequencing according to the instructions of 454 Life Sciences (Branford, CT).
The fusion primers used for PCR amplification were designed for amplicon sequencing according to the instructions of 454 Life Sciences.
The primers used for PCR amplification were designed for TruSeq sequencing according to the recommendations of Illumina.
The primers used for PCR amplification were designed for TruSeq sequencing according to the instructions of Illumina, with the 3′-sequencing adapter containing a barcode specific for each library.
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