Sentence examples for for sequence confirmation from inspiring English sources

Purified plasmids were submitted to the UNMC core facility for sequence confirmation.

For sequence confirmation, two independent digests are performed using enzymes which cut at opposite ends of the spacer.

The spacer is designed to separate the arms of the palindrome for efficient cloning, to provide restriction sites for linearization of the construct and to provide unique primer binding sites for sequence confirmation of the insert.

Atwell et al. [12] genotyped 107 lines of Arabidopsis with the 250K array to determine which SNPs were polymorphic between two given lines to a high degree of confidence, using quality ensuring measures such as multiple prior data sets for sequence confirmation, the filtering of bad arrays and bad SNPs using mismatch rates, and removing non-binary SNPs.

All PCR products were subcloned for sequence confirmation.

This finding reinforces the need for sequence confirmation of PCR products.

The recombinant plasmids were first transformed into competent cells of E. coli DH5α for DNA sequence confirmation, and then transformed into E. coli BL21 (DE3) for cytoplasmic overexpression of OleTJE and Tll.

For further sequence confirmation, the fragments were sequenced using the M13 forward and gene-specific primers.

Following transformation into E coli four colonies from each were picked for DNA sequence confirmation of the insert.

The PCR product was purified with Wizard PCR preps DNA purification kit (Promega, UK) according to the manufacturer's instructions and ligated into pCR2.1TOPO (Invitrogen, UK) for nucleotide sequence confirmation (MWG Biotech, Germany).

More important, the nanomicroarray assay can detect emerging and reassortant viruses, and those samples can be sent to centralized laboratories that perform the NGS assay for final sequence confirmation.