These five additional polymorphic markers were likely not polymorphic between BAT 93 and Jalo EEP 558 due to residual heterogeneity in these two lines and the DNA used for the sequencing was a separate extraction from the DNA that was used for the GoldenGate analysis.
For example, separate extractions with water, alcohol, and organic solvents can yield different metabolite profiles [ 62].
We designed separate extraction components for different types of temporal relations, utilizing a novel hybrid system that combines machine learning with a graph-based inference mechanism to extract the temporal links.
For the estimation of ascorbate peroxidase (APX; EC 1.11.1.11) activity separate extraction was carried out with the extraction buffer solution containing 100 mM phosphate buffer (pH 7.0), 0.1 mM EDTA, 1.0 mM ascorbate, and 1 mM DTT.
In the aDNA extraction step, 13 of the samples (out of the 26 samples which were successfully amplified for the 523-bp mtDNA D-loop sequence) (Table 1) were extracted several times, and at least two PCR-reactions were performed for separate DNA extractions to amplify and sequence each overlapping mtDNA D-loop fragment at least twice.
For samples from naturalized populations in Florida (n = 188), leaf tissue was taken for separate DNA extractions from 7-27 individual plants per site from 12 counties throughout the state.
The extent of monoterpene diffusion was evaluated by placing a series of tangential cryosections on 4 μl drops of 10 mM DTT, pipetting up and down, and then collecting the aqueous phase and tangential sections for separate metabolite extractions.
Separate extractions were performed for each of 60 F1 hybrids, which were pooled before sequencing forming two biological replicates (each n = 30).
After collecting the material, seeds of Libidibia ferrea were separated for extraction.
We consider the design feasibility of using ionic liquids as solvents in liquid liquid extractions for separating organic compounds from dilute aqueous solutions.
