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Note that all of these approaches propose analytic solutions for selected target designs, while our method numerically optimizes for phase patterns given arbitrary target designs.
Quantitative PCR was performed using specific primers for single var genes (Salanti51, Supplementary Table S1 or specific primers for selected target genes with Power SyBr Green PCR Master Mix (Thermo Scientific).
The epitopes recognized by antibodies from patients who have quinine-induced thrombocytopenia have been characterized for selected target molecules.
(D ) Gene expression reflects the crosstalk observed at the signaling level, as verified by qPCR for selected target genes.
The quality of the gilthead sea bream oligo-microarray data was also confirmed after qRT-PCR validation of expression results for selected target genes.
To validate the findings of changed gene expression on protein level, Western Blots were performed for selected target genes whenever appropriate antibodies were available.
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Abridged pathway network for selected targets.
(d) Changes in 5hmC after a 6-h SD for selected targets.
Nanoparticles have been integrated to optical sensing and imaging techniques to achieve advanced detection performance for selected targets.
RT-qPCR was performed for selected targets using SYBR Green Master-Mix (Bio-Rad) to validate the microarray results.
(A ) Sample RT-PCR gels showing aberrant splicing ratios for selected targets important for neuronal development and function.
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