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Comparison of the specificities for selected substrates in wild-type hPEPT1 and hPEPT1Q300K (Fig. 6) clearly showed that the Q300K mutation induced affinity for Asp-Ala in hPEPT1Q300K.
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Automated analysers allow high-throughtput measurements, however they have limitations in two situations: (a) some of them do not have available the wavelengths required for the measurement of selected substrates, (b) contamination can occur when paroxon or other organophosphorous compounds are used as substrates as previously discussed.
Descriptors involved with the net surface charge and energy level of substrates seem to be the common integral factors for defining binding specificity of selected substrates to BCRP polymorph.
Various positions for establishing Pt atom on the selected substrates have been considered and full structural optimization was carried out for all selected systems.
Hence, selected substrates were analysed for determining the TEs contents and other properties that are connected with TEs activities.
We grew at 900 °C for 15 min. The deposition time was selected in order to achieve a film that covered the whole substrate that did not experience peeling or delamination from the copper substrate for the selected substrate temperature.
The relative affinity of αB crystallin for itself and selected substrate proteins explains the functional significance of the dynamic subunit model for sHSP assembly in regulation of sHSP structure and function [37].
The cells were grown on the selected substrate for 1 week (37 °C, 5%% CO2).
An analysis of multiple shRNA intermediates generated from several reagents revealed the complexity of shmiR processing by Drosha and demonstrated that Dicer selects substrates for further processing.
OGG1 and UNG, for instance, were proposed to pre-select substrates by establishing superficial base contacts through conserved residues in close proximity to the mouth of their catalytic pocket.
Finally, we directly detected in vivo phosphorylation at Cdk motifs for selected putative substrates using mass spectrometry.
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