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The activated ClO2 solution was then diluted in water to a total concentration of 500 μg/mL, followed by subsequent dilutions to approximately total concentrations of 200 μg/mL, 100 μg/mL, and 50 μg/mL for selected experiments.
For selected experiments, iodo acetate was added by sterile filtration to the sterilized growth medium giving a final concentration of 1 mM.
Global proportions were calculated for selected experiments.
Strength values for selected experiments are shown.
For selected experiments, cells were treated with 0.5 μg/ml actinomycin D (#A9415, Sigma-Aldrich) for 2 h.
Isotype IgG controls were purchased from the respective vendors (as above) or from Sigma for selected experiments.
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We present a method to use samples from simulated predictive distributions for selecting experiments useful for model selection.
Finally, for select experiments we utilized transgenic Luciferase+ CD-1 mice.
In addition, for select experiments mouse ASCs were derived from the inguinal fat pads [34] of transgenic CD-1 mice with constitutively active Firefly luciferase in order to quantify cell persistence upon in vivo engraftment.
For selected optimization experiments only LTQ analyzer was used to register MS spectra.
Instead, we performed duplicate experiments for selected conditions.
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