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Sampling then used PPS methods again for secondary sampling units and simple random sampling from household lists for tertiary sampling units.
For bird-level seroprevalence calculations over the entire study period, we accounted for repeated measurements within the same birds by specifying the individual bird as the third level of sampling and incorporating the number of duck farms per village as the finite population correction factor for secondary sampling units.
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The rank K = 3 clustering was further tested for significance by permuting sample labels for secondary samples independently for each chromosome.
For this study to have the security of a probability sampling method, we will use the compact segment sampling method for selecting secondary sampling units [ 18].
Sampling used proportional population to size methods for primary and secondary sampling units, followed with simple random sampling from household lists for tertiary sampling units.
To control for secondary immunoglobulins, samples were processed with omission of the primary antibody.
For secondary drying, samples were warmed by increasing the shelf temperature at a rate of 0.2°C/minute to the designated secondary drying temperature to be tested and maintaining the shelf at this temperature for 360 minutes.
For the MiSeq™ (Illumina) assays, all 36 secondary samples and for the GS Junior a different set of 18 samples were used.
Paired observations were extracted from the forest cover map and the reference data for a random selection of 50 secondary sample units (SSUs) in the form of pixels within each PSU.
This PSN must be repetitively unambiguous for a patient, so that researchers can correlate and utilize for secondary use several samples from the same patient (e.g. tumor and control sample).
Given participant perspectives on future use of samples in African settings, it is important to allow participants to express their choice regarding consent for secondary use of samples.
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