Nowadays, experimental designs for secondary modelling within the field of predictive microbiology are mostly arbitrary or based on factorial design.
VIP-values are also used for variable selection for the generation of secondary models.
The WinBUGS code for our primary model and the Excel spreadsheet for our secondary model are included in the Appendices.
For the secondary model, it was proposed to model the effect of temperature and initial nitrogen content on the kinetic parameters of the primary model with an Arrhenius relationship.
For our secondary model, ie, the Microsoft Excel spreadsheet, we used all available proportion and average cost estimates as raw inputs.
For a suitable prediction of ricin inactivation, a secondary model for every interim product in the food chain would be necessary.
Finally, significant correlation coefficients were found between ergosterol and colony diameters, suggesting that both parameters may be useful for primary modelling and thus for subsequent secondary modelling.
As a secondary model for analysis, the final 20 items were also subjected to a traditional item analysis.
Another known prognostic factor, T-stage (T1/2 vs T3/4), did not contribute to predicting PFS in secondary models accounting for ERCC1 assays and randomisation stratum (models not shown).
Figure 2 shows a typical example for secondary structure modelling in the topology string.
Considering the fruitful results of this method in terms of accuracy and running speed, an extension of the approach, hereby named TS-AMIR, is introduced to be used for secondary structure modelling in a topology string with the ultimate goal of developing a structural protein alignment tool.
