Sentence examples for for second plate from inspiring English sources
Chromo4 real-time PCR machine (Biorad) was used with the following programme: 95 °C for 10 minutes, 95 °C for 10 seconds, 60 °C for 15 seconds, 72 °C for 15 seconds, 75 °C for 1 second, plate read, repeat from the second step 39 more times, 72 °C for 5 minutes, melting curve from 55 °C to 95 °C, read every 0.2 °C, hold for 1 second and finally 75 °C for 5 minutes.
The success rate of QPCR based on the amplification plots was high: 88.5% for the first plate [see Additional file 6] and 90.6% for the second plate [see Additional file 7].
Another day, a diner idly wished for a second plate of bread, and seconds later it appeared.
It was when I was going to the long, stainless-steel bar for a second plate, with a little turkey in me, that I saw my family from a distance.
For my second plate I dialed things back.
Got to eat something for your second plate.
For the second plate cells are dissolved followed by CFU determinations on BHI agar plates.
Each extension was followed by data collection at 72°C and a short incubation step at 78°C (1 sec) for a second plate read closer to the melting point.
Preliminary analyses of these data allowed for the estimation of the number of additional GS 20 sequencing lanes (three for Nandina, five for Platanus) on a second plate that were necessary to obtain approximately 20× coverage for each genome.
But when another person asked if we could have a second plate for the table, he immediately declined.
