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Each primer mix contained 200 nM FAM-labeled probe, 300 nM of each primer and 1x Perfecta qPCR Supermix (Quanta BioSciences Inc .. Expression of YARS, a t-RNA synthetase, was used for sample validation and normalization of expression.
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The same method is then used to predict classes for new samples (validation set).
Thus, they can be used for out-of-sample validation.
We estimate the VAR model with 16 years of hourly historical data and use two additional years of data for out-of-sample validation.
An additional data value, the percentage of the diagnosed population on treatment, was used for independent out-of-sample validation.
We used a split-sample validation technique for statistical analysis, as described previously [ 22].
Good practice for qualitative research, including theoretical sampling, validation and conceptual analysis was followed [ 32- 35].
We applied a split-sample validation technique for our statistical analysis.
The authors thank Priya Chockalingam at Pfizer, Cambridge, MA, for sharing information on their aggrecan capture BC-3 ARGS ELISA with us, and for analyzing samples for validation.
Linear discriminant analysis was performed on spectra of 228 samples which were randomly divided into 138 samples for calibration and 90 samples for validation.
Our analysis on the larger Italian cohort showed that the "leave-one-out" methods correlates with results obtained using the traditional ANN approach (2/3 of samples for training, 1/3 of samples for validation).
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