An outline of methods for sampling specific target populations is provided in the second section.
A number of techniques are available for sampling specific plant cells, including fluorescence-activated cell sorting (FACS) [ 21] and laser capture microdissection (LCM) [ 22, 23].
However, our discovery of an unexpected off-target site highlights the necessity for sample-specific gRNA design, especially in therapeutic applications of CRISPR/Cas9.
Genotypic and phenotypic susceptibility scores were calculated at baseline (week 0) and week 2. Virus from 500 1,400 µL of plasma was pelleted by centrifugation (17,000×g, 1 hr, 4°C), resuspended in 140 µL of plasma, and extracted using the QIAamp viral RNA mini kit according to the manufacturer's instructions (Qiagen, Valencia, CA; see Supplementary Methods S1 for sample-specific plasma volumes).
See Supplementary Table S1 online for sample-specific details.
Each sample was assigned a different forward/reverse index combination for sample-specific labelling.
Let denote the random variable for sample-specific estimated isoform usage, whose observed value is.
Normalized coverage is further corrected for sample-specific capture bias due to GC-content and bait probe hybridization likelihood.
As before, this artificial codebook matrix can be used for sample-specific component reordering and visualisation (see Fig. 3B).
Because of insufficient serum volume for sample-specific lipid measures, concentrations of the ΣPCB4 were measured on a wet-weight basis.
The computational methods described here can be separated into four steps: raw coverage normalization, correction for sample-specific coverage biases, CNV calling, and partially-mapped read analysis.
