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The following steps for sample preparations are same as mentioned in "Immunostaining" section.
For sample preparations, 5 ml of aniline blue solution, 0.001 % (w/v) in 50 mM phosphate buffer pH 7.0 was mixed with 50 mg of sample and allowed to stand at room temperature for 15 min.
For sample preparations, the antisense strand of firefly luciferase sequences were obtained from Ambion (Austin, TX).
Illumina mRNA-SEQ kit was applied for sample preparations and RNAseq libraries were generated with Illumina Genome AnalyzerIIκ.
In the next sections, we will briefly review the theoretical background of EMD and explain the details for sample preparations, experimental procedures, and data analysis.
Therefore, the development of novel, high-throughput, sensitive technologies for sample preparations is a prerequisite for the successful detection of the small number of exfoliated cells or of the small amounts of DNA, RNA and proteins in these cells.
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MS prepared documents for sample preparation and instrument analysis.
KBr powder was used for sample preparation.
An improved design was used for sample preparation.
A microwave-assisted digestion method was used for sample preparation.
We thank E. Soballa for sample preparation and SEM imaging.
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