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Minimum required sample size for our study was 5 for both groups, for the following parameters: number of groups = 2, type I error rate α = 0.05, statistical power 0.8, average value (standard deviation): for sample one AVE = 68.9 (24.3) cm; for sample two AVE = 49.4 (46.4) cm.
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As pressure increases, we observed two obvious yield points for samples, one at P = 3 GPa in the elastic deformation stage and the other at P = 4 GPa.
For Sample 1, one blood sample was missing and could therefore not be analysed for PFAA content.
Two PCRs were performed for each sample, one for wild type MYD88 and one for possible detection of mutated MYD88 by using primers as previously described [ 18].
The MEMS designed for analyzing a spent fuel solution consists of three microchannels: one channel for the sample, one for a reference solution, and one is the blank.
Six PCR amplifications were performed for each sample; one for each of the six exons of interest.
Two separate ligation reactions were performed for every sample, one reaction for each linker cassette using 15 U T4 ligase (30 U T4 ligase HC from Thermo Scientific).
For each sample, one section was prepared for routine HE staining and others for immunohistochemical staining.
For each sample, one quarter of the PCR product was used for constructing a barcoded Illumina DNA library.
For each sample, one half of the powder was kept at -80°C for RNA extraction.
Then, the reduce process selected for each sample one of its neighbors randomly to interpolate with it.
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