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For routine experiments, an X20 Plan Apo objective under differential interference contrast (DIC) was used.
However, a lack of methods for quantitative analysis limits the usage of spheroids as models for routine experiments.
Our dose response curve confirms these findings and for routine experiments 1 pg/ml or 10 pg/ml was used.
However, the survival of dechorionated embryos is extremely low during electroporation, making the method inapplicable for routine experiments.
Since the response to asialofetuin served as a negative control, a wash step was not warranted for routine experiments.
As a result we did not use these dyes for routine experiments, but they could be useful when cell yield is paramount.
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Since this novel catheterization technique is repeatable, is easy to perform and facilitates semen preparation protocols, it may be preferable for routine IVF experiments with fresh spermatozoa.
We recommend for routine genotyping experiments using those probes that exhibit higher hybridization intensities from the reference genome (e.g., B>M probes) for initial mapping applications because they have higher validation rates.
For these reasons, qPCR using a simple DNA dye is a popular choice among academic laboratories for routine PCR experiments.
Moreover, this method provides less contamination and lower cost, both of which are benefits for routine biological experiments.
For routine laboratory experiments, isolates were maintained on a combination of glucose minimal medium (GMM) (Shimizu and Keller 2001) and Champe's medium (Champe and El-Zayat 1989).
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