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The enhanced performance for relative intensity noise (RIN) reduction based on reflective semiconductor optical amplifiers (R-SOA) has been investigated theoretically by comparison with conventional transmission SOA.
Western blots were analyzed for relative intensity of BCA2 protein levels, normalized to β-Tubulin.
To control for relative intensity, experimental and control samples were stained in the same dish and imaged under identical conditions.
After 20 min exposure, the membranes were scanned using a phosphorimager (BioRad Molecular Imager Fx; Bio-Rad Laboratories, HerCAles, Candanalyzedyzed for relative intensity of expression.
Means ± SEM for relative intensity of in situ signals after normalization to the level in control DRGs is as follows: Control, 1.00 ± 0.16; Ngf -/-Bax -/-, 0.73 ± 0.12.
The trend for relative intensity (normalized to the highest value) is similar to the straight-only SLIM arrangement shown in Figure 5B, with ion transmission optimized for guard bias from ∼7 to 12 V.
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Thus the equation for relative intensities b in decibels may be written b = 10 log10 (I/I0).
While our software does not specifically account for relative intensities of the reflections, which would have provided information about molecule placement within the lattice, we can account for lattice symmetries by considering different space groups.
For calculation of relative intensities the pixel intensity was normalized to the fluorescent signal of elav genotype.
For calculation of relative intensities, all pixel intensities in the selected areas were normalized to the fluorescent signal/pixel intensities of the elav genotype.
To minimize the effect of experimental parameters fluctuations, emission line of carbon at 2478 Å was used for normalization of relative intensities of all spectral lines.
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