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One such approach involves inducing mitotic recombination and selecting for recombinants using a chromosome carrying a dominant female sterile mutation (Perrimon and Gans 1983).
The RT-cassette, inserted at the target site by an initial recombineering step using positive selection for recombinants, is replaced with the desired sequence in a second round of recombineering using negative selection.
However, incompatibilities can be beneficial in the introgression approach, as they select for recombinants between the incompatibility and the QTL and can therefore increase the effective recombination rate during the backcross.
Each dataset was analyzed for recombinants using RDP [72] and recombinants were excluded from the analysis.
If no phenotypic screening system for recombinants is available, a novel restriction site can be used for initial colony screening.
The linear DNA fragment was electroporated into B5055+pKD46 or B5055nm+pKD46 and grown at 37°C on chloramphenicol to remove pKD46 and select for recombinants respectively.
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The conference led to guidelines for recombinant DNA research, enabling experimentation to continue.
But they said more research was needed before the drug, known as rNAPC2 for recombinant nematode anticoagulant protein c2, could become an accepted treatment for Ebola.
Apparatus used for recombinant glucagon and costs for 1litre expression.
Figure 4 The circular dichroism results for recombinant proteins.
Yeast is a widely used host for recombinant protein expression.
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