I wasn't really ready for the isolation, for the daily grind of it.
The extract was kept in the refrigerator (4 °C) ready for chromatographic isolation of compounds.
After three days, transduced cells were split into gelatine-coated six-well plates and maintained in ESGRO Complete Plus Clonal medium [Millipore] with regular medium changes until colonies appear and are ready for clonal isolation and expansion.
The samples were stored at -80°C until ready for RNA isolation.
Magnetic particles coated with alginic acid under high PEG and sodium chloride concentration were used for the isolation of PCR-ready bacterial DNA from various dairy products.
The field of plant molecular biology is therefore at a disadvantage, especially when an automated high-throughput system for the isolation of PCR-ready genomic DNA is required in population genetics, species identification, biodiversity investigation, selection screening, food control and plant biotechnology.
Samples were centrifuged, aliquotted and then stored at −80 °C until they were ready to be processed for RNA isolation.
Total RNA was isolated from the five prostate cancer cell lines, including the si- PRKC-ζ -a -PC-3M T1-6 knockdown cells, using RNeasy Mini Kits (Qiagen, Crawley, UK), a ready-to-use reagent for the isolation of total RNA from cells and tissues, following the manufacturer's recommendations.
The TRIzol® LS reagent used herein is a ready-to-use solution for isolation of total RNA from liquid samples of different origins (TRIzol® LS).
The diagnosis of mastitis was confirmed by milk cultures that were plated onto ready-to-use Baird Parker (selective medium for staphylococci isolation) and Columbia Blood Agar for isolation of streptococci, staphylococci, corynebacteria and related bacteria.
