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Therefore, a newly designed dUTPase-negative ILTV vector was used for rapid insertion of the H5-hemagglutinin, or N1-neuraminidase genes of a recent H5N1 HPAIV isolate.
Rapid advances in molecular biology should allow for rapid insertion of this 4-gene expression cassette in industrial yeast strains to improve production, not only of 1st and 2nd generation ethanol production, but also of other renewable fuels or chemicals.
There is a growing body of evidence to support this model (reviewed in [ 25]); however, although there is evidence for rapid insertion of TRPCs from a sub-plasmalemmal pool using TIRF (total internal reflection fluorescence) microscopy [ 5], the possibility of contributions by other intracellular pools to this process has not been investigated.
However, the initial cloning step may prove useful in many cases, as it may provide unique restriction sites for rapid insertion into other retroviral vectors containing convenient markers (i.e. alternate antibiotic resistance markers, GFP) or other context-specific features (ie., knock-in Gene A, knock-down Gene B).
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Since the gold bands where the immunoreactions took place have to be inserted in the flow system for the detection, a novel single flow cell that enabled their rapid insertion was specially designed.
Temperature profiles were made using a thermocouple and the rapid insertion technique.
The predictions of "soot" concentrations from numerical simulations for nitrogen-diluted, ethylene/air flames are compared with laser-induced incandescence and Raman spectra observed from samples thermophoretically extracted using a rapid insertion technique.
For the expression of human intact IgG antibodies, we have constructed a set of baculovirus expression vectors designed to facilitate rapid insertion of heavy and light chain genes of Fab or scFv antibodies derived from phage display antibody libraries.
The relatively late and then rapid insertion into the global economy catalyzed a number of changes.
The rate law for ROCOP using 1, shows a zeroth order in carbon dioxide (1 40 bar), which is consistent with the rapid insertion of CO2 into zinc alkoxide bonds.
For initial gap closure, Single Primer Amplification of Contig Ends (SPACE), which is similar to the single-primer PCR procedure for rapid identification of transposon insertion sites, was used [ 21].
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