Sentence examples for for protein reduction from inspiring English sources

The other is for protein reduction.

A mini incubator from Labnet was used for gel washing, for protein reduction and for protein alkylation steps.

A volume of 50 μL denaturation buffer (8 M urea, 400 mM NH4HCO3, Sigma) was added, followed by the addition of 10 μL DTT (45 mM, Sigma) and incubation at 55°C for 15 min for protein reduction.

For protein reduction gel spots were incubated for 60 min with 10 mM dithiothreitol in 25 mM Ambic at 37°C followed by alkylation at room temperature in the drack with 55 mM iodoacetamide in 25 mM Ambic.

After being washed three more times with 25 mM ammonium bicarbonate and 50%acetonitrile/255 mM ammonium bicarbonate, the gel fragments were placed at 56°C for 1 h in a solution containing 10 mM dithiothreitol, 55 mM iodoacetamide, and 25 mM ammonium bicarbonate for protein reduction and alkylation.

After the first-dimensional isoelectric focusing, IPG strips containing proteins were treated for 15 min with gentle shaking in the equilibration buffer (6 M urea, 30% glycerol, 2% SDS, 50 mM Tris, pH 8.8) containing 2.5% tributyl phosphine (TBP) for protein reduction followed by protein alkylation with 3% iodoacetamide (IAA) to replace TBP in the buffer for another 15 min.

There are several possible explanations for VAPB protein reduction in ALS8 patients.

Three sterile sorts were performed to acquire a polyclonal population of cells exclusively expressing GFP that was subsequently examined for TDAG51 protein reduction by western blotting.

Furthermore, a possible increase of PHD activity upon BAY 87-2243 treatment could also be excluded as the underlying cause for the HIF-1α protein reduction, as the compound was inactive in a biochemical PHD activity assay.

In this paper, we describe a simple method for the determination of heme protein reduction potentials.

We describe a simple method for the determination of heme protein reduction potentials.