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The proposed technique provides a simple and robust method for protein patterning.
For alkylsilanes, degradation of the adsorbate molecule provided a facile means for protein patterning.
The first requirement for protein patterning is thus an effective means to control nonspecific adsorption.
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[8 and 2 μg protein were loaded into the gel for protein patterns and activity staining, respectively.] Lane 1: crude enzyme extract; 2: top phase of effect CE; 3: top phase of effect pH; 4: top phase of effect NaCl addition; 5: top phase of repetitive ATPS; M: molecular weight protein makers.
The gels were silver stained (Heukeshoven and Dernick, 1988) for protein pattern analysis or processed for western blotting.
Each sample was run in two PAA gels: one with entrapped peroxidase for zymography and another peroxidase-free for protein pattern.
It is conceivable that microdispensing can be utilized for the creation of protein patterns for guiding neurites to obtain in vitro neural networks, in tissue engineering or rapid screening for guiding proteins.
Zhou, F., Chen, Y., Felner, E, I., Zhu, C. & Lu, H. Microfluidic auto-alignment of protein patterns for dissecting multi-receptor crosstalk in platelet.
To this end leaf proteins were extracted from L-, N-, L→H- and N→H-light samples after 6 h of treatment and subjected to 2D gel electrophoresis with silver staining for sensitive visualization of protein pattern.
In our study, a surface-enhanced laser desorption/ionization time of flight (SELDI-TOF -based ProteinChip SELDI-TOF -basedfor rapid identification of protein patternSELDI-TOF -based
In recent years, there has been a strong demand in the biological community for multiplexed protein patterns for the control and study of cellular growth at subcellular and cellular length scales.
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