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This is the case for protein localization, where proteins can be localized to many distinct compartments, e.g. nucleus, ER, Golgi, cytoplasm, membrane and mitochondria.
Post-translational modification of proteins by the lipid palmitate is critical for protein localization and function.
Feilmeier, B.J., Iseminger, G., Schroeder, D., Webber, H. & Phillips, G.J. Green fluorescent protein functions as a reporter for protein localization in Escherichia coli.
Hopkins, C., Gibson, A., Stinchcombe, J. & Futter, C. Chimeric molecules employing horseradish peroxidase as reporter enzyme for protein localization in the electron microscope.
We review measurements of microtubule‐based motility as well as measurements for protein localization and protein segregation in vitro.
These plasmids allow the creation of green or red fluorescent protein (GFP or RFP) tagging vectors for protein localization studies, as well as split-yellow fluorescent protein (YFP) tagging vectors for bimolecular fluorescence complementation (BiFC) analyses.
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Dinh, T. & Bernhardt, T. G. Using superfolder green fluorescent protein for periplasmic protein localization studies.
Therefore, this assay system could represent another valuable tool for determining protein localization.
The in vitro protein import experiment is one of the most important techniques for determining protein localization.
This quantification of the surface geometry and fluorescence distribution allow for a more precise measure of 3D cell shape and is a useful tool for measuring protein localization and the mechanisms of bacterial shape control.
Currently there is no PRPF3-deficient mammalian cell line for studying protein localization.
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