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Genomic DNA was also purified from an aliquot of cell pellets used for protein extraction.
Exosomes used for protein extraction were resuspended in 20 30 μl of RIPA lysis buffer.
Two OD units (Optical Density units) were harvested for protein extraction and three OD units for total RNA extraction.
The methods for protein extraction, followed by western blot analysis, were based on protocols from previous studies22,48.
Uteri were dissected and collected in RNA later, 4% neutral buffered formalin for paraffin embedding and snap frozen in liquid nitrogen for protein extraction.
(A) Detergents for protein extraction and purification.
Mature seeds harvested from T3 homozygous plants were used for protein extraction.
Mycelia were then collected and washed twice with sterilized water for protein extraction.
Large volumes of treated process water are required for protein extraction.
After 8 days, the second leaves were cut down for protein extraction.
According to the manufacturer's recommendations, 5×108 Hcells cells were used for protein extraction.
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