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Our data show that mutation of a factor required for proper processing and post-transcriptional regulation of histone mRNAs alters chromatin structure.
Moreover, phosphorylation of these sites is functionally important, but not essential, for resistance to replication stress and for proper processing of proteins in the cell.
This would be consistent with the view that cholesterol accumulation in NPC cells represents a dynamic and mobile storage pool [40], with presumably, redistribution of stored cholesterol allowing secondarily for proper processing of GSLs.
Although the ITS are not incorporated into the mature rRNA, they encode signals for proper processing of the rRNA transcripts, which itself depends on the secondary structure of ITS RNA [3].
Our model also provides a rational explanation for membrane targeting of bacterial autotransporters, which possess long signal peptides: These are in accordance with our NtraC model, where the C-domain alone is sufficient for transport to the inner membrane but for proper processing the complete signal peptide is required [45].
The discrepancy between IL-8 transcriptional and protein levels could be due to the fact that IL-8 mRNA undergoes extensive post-transcriptional regulation [42], and the reliance of 3O-C12 onon-canonicalal signalling pathways [43] is insufficient in some cell types to induce the cellular machinery required for proper processing and secretion of this cytokine.
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Finally, based on these relations, a laser cladd processing map was designed to use as a guideline for the selection of proper processing parameters for a required coating.
These relations lead to the design of a laser cladding processing map that can be used as a guideline for the selection and further tuning of proper processing parameters for laser cladding of extensive layer.
Statistical correlations led to the design of the laser cladding processing maps that can be used as a first guide in the selection of proper processing parameters for laser clad coatings of the required thickness.
Statistical correlations led to the design of the laser cladding processing maps that can be used as a first guideline in the selection of proper processing parameters for laser clad coatings of the required thickness.
The expression yield of the deglycosylated mutants was not significantly affected, indicating that glycosylation at Asn-201 was not required for a proper processing and secretion of this protein by P. pastoris.
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