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A reference sample was labeled with Cy3 for proper normalization.
Although the program recommended Vn/ n + 1 values of less than 0.15 for proper normalization, this requirement is not absolute.
For proper normalization of the source PPI datasets to the UniProt identifier level, it was also important to consider the continuous updating of biological information, since it can lead to changes in the annotation of protein identifiers and in their associations at other molecular levels.
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Different transcript quantification methodologies exist, all of which rely on utilization of proper normalization techniques.
For the proper normalization of the expression levels in qPCR studies, we selected new control genes.
The relative contributions at each level can be specified after proper normalization for the number of firms.
One prerequisite for validating RT-qPCR data is proper normalization with respect to stably expressed endogenous reference genes.
Thus, proper normalization strategy is necessary for reliable quantitative information to be extracted from this variable system.
A number of strategies have been proposed for proper data normalization in qRT-PCR [ 16- 18].
For interpretation of quantitative mRNA measurements in clinical samples, a proper normalization is an essential component of reliable qPCR assays, which is necessary to correct for sample differences in cellular input, RNA quality and real-time efficiency [ 23].
Then, generally, a proper normalization algorithm is used to estimate expression levels for comparative analyses.
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