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Isolation, by purification by immobilized metal-affinity chromatography, of full-length rRTF was facilitated by engineering a (His 6 tail on its C-terminus, which maximizes the selection of rRTF with intact transmembrane and cytoplasmic domains, critical for proper activity.
Thus eIF3f plays a major role for proper activity of mTORC1 to regulate skeletal muscle size.
Last, they may be necessary for proper activity of their sense partners, as shown for the Xist/Tsix pair [51].
This argues for conservation of function, as both fly and vertebrate Nkd/Nkd1 appear to require association with the plasma membrane for proper activity in antagonizing Wnt signaling.
Previous analyses of the stripe 2 and stripe 3 enhancers in transgenes demonstrated that spacing between the elements, but not their ordering relative to the promoter, was required for proper activity [31].
Therefore the mutual interplay between Zif and aPKC is critical for proper activity of aPKC during neuroblast asymmetric division.
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Biotin is crucial for proper activities of carboxylation enzymes involved in BCAA and cysteine catabolism and TCA cycle anaplerosis (i.e., propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, pyruvate carboxylase).
This activating phosphorylation is essential for the proper activity level of MPF.
The binding might be too strong for purposes of dynamic regulation which is required for the proper activity of HMGA2.
Astrocytes in the white matter extend thin processes that contact axons at the nodes of Ranvier, where they are presumed to participate in the regulation of homeostatic and metabolic functions necessary for a proper activity of axons [7].
Moreover, myosin, as one the major actin regulators, is totally dependent on Ca2+ for its proper activity [ 24].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com