Isolation, by purification by immobilized metal-affinity chromatography, of full-length rRTF was facilitated by engineering a (His 6 tail on its C-terminus, which maximizes the selection of rRTF with intact transmembrane and cytoplasmic domains, critical for proper activity.
Thus eIF3f plays a major role for proper activity of mTORC1 to regulate skeletal muscle size.
Last, they may be necessary for proper activity of their sense partners, as shown for the Xist/Tsix pair [51].
This argues for conservation of function, as both fly and vertebrate Nkd/Nkd1 appear to require association with the plasma membrane for proper activity in antagonizing Wnt signaling.
Previous analyses of the stripe 2 and stripe 3 enhancers in transgenes demonstrated that spacing between the elements, but not their ordering relative to the promoter, was required for proper activity [31].
Therefore the mutual interplay between Zif and aPKC is critical for proper activity of aPKC during neuroblast asymmetric division.
The simpler architecture of FTase and GGTase I, and their independence from further components for proper activity suggests that they preceded RGGTase.
For example, the usage frequencies of amino acid cognates in regular primate mitochondrial ORFs coevolve with various antisense tRNA properties that are required for proper activity in translation [ 24].
In recent work with mammalian cells, Stavreva et al. found that complexes associated with pre rRNA processing factors are ubiquitinated and hence labeled for processing by the proteasome, a step essential for proper activity in ribosome maturation.
Structural analyses of Paak1 and PaaK2 in complex with either ATP or phenylacetyl adenylate revealed several important residues for proper activity (PDB codes: 2Y27, 2Y4O, 2Y4N; Law and Boulanger 2011).
