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31 To determine whether the aberrant increase in uninfected Iba-1+ brain macrophages, including microglia, in the uninfected areas was due to in situ proliferation or recruitment from other sites, a BrdU incorporation assay for proliferating cells was performed at 5 dpi (Fig. 4B).
The methylation distribution of these same CpGs for proliferating cells (left).
PCNA, a marker for proliferating cells, was negatively correlated with MeHg dose, with a significant reduction in cell number in the forebrain and spinal cord of exposed embryos by tadpole stages.
Although the surface of this template is more hydrophobic by comparison with the bare Ta surface, the cells on this material were successfully incubated, indicating that the collagen coated Ta might be used as the buffer layer for proliferating cells in hydrophobic biomaterials.
(C) Immunostaining for proliferating cells: Ki67 (green), pH3 (red, mitotic marker).
For proliferating cells, fresh medium was provided.
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Hepatocellular proliferation was analyzed by immunostaining for proliferating cell nuclear antigen (PCNA, Neomarkers, clone PC 10, dilution 1∶500).
These samples were analysed for proliferating cell nuclear antigen (a cell proliferation marker), nucleophosmin (NPM, a direct CDK2 substrate) and Ki67 as described previously (Mahadevan et al, 2011).
To confirm that IGF1 induces arrest in irradiated salivary glands, we measured proliferation by staining tissue sections for proliferating cell nuclear antigen (PCNA).
In animal tumor models with high-expression levels of lymphatic vessel growth factor VEGF-C[ 9] or VEGF-D[ 10], the endothelial cells along lymphatic vessels show proliferation and a positive stain for proliferating cell nuclear antigen (PCNA).
To assess proliferation in the glands, we stained for proliferating cell nuclear antigen (PCNA) (Fig. 6a-c).
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