The GeneChip Mouse Genome 430 2.0 Array, which represents >39,000 transcripts and variants, was used for profiling analysis.
We also analyzed the transcriptional consequences of knocking out the Akr1b8 and Rgs2 genes; unfortunately tissues were not available from the Emr1−/− mice for profiling analysis.
To obtain a comprehensive portrait of miRNA expression during preimplantation development, we collected embryos at the zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst stages for profiling analysis.
Each of the six cDNA preparations was divided into three aliquots (technical repetitions) to provide nine repetitions per genotype for profiling analysis.
The analysis results were very similar to the ones obtained with the 50 mm guard column with results ranging 92.0-101.8 % for DH1, 98.2-101.0 % for DH1Δ acrB and 88.8-91.6 % for BW25113 profile analysis compared to the 300 mm column (Fig. 2b).
The blood samples were taken from abdominal aorta and centrifuged at 2500 rpm for 15 min at 4°C to extract the serum for hormonal profile analysis.
For plasmid profile analysis, plasmids were resolved by electrophoresis at 110 V for 3 hours in 0.8% wt/vol agarose.
After maturation by poly(I/C) for 48 h, cell culture supernatants were collected and stored at −20 °C for cytokine profile analysis.
Lee, S. H., Goddard, M. E., Wray, N. R. & Visscher, P. M. A better coefficient of determination for genetic profile analysis.
