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Consequently, some form of data normalization is required for processing microarray data in order to allow intercomparison of raw data.
In the absence of a "gold standard" for processing microarray data, these statistical threshold cutoffs serve to maintain a reasonable and consistent level of data quality across all studies analyzed within NextBio and are commonly adopted in the literature [43] [45].
[ 19] which has been designed for processing microarray data.
Several probe set algorithms were available for processing microarray data.
Common approaches for processing microarray data seem adequate for our approach.
Similar(55)
Isolated RNA in DEPC-treated water was then immediately frozen at −80°C for 24 h and shipped on dry ice to ArrayStar (Rockville, MD, USA) for mRNA processing, microarray hybridization, and probe expression normalization.
We thank the ONPRC microarray core for processing the microarrays.
Du, P., Kibbe, W. A. & Lin, S. M. lumi: a pipeline for processing Illumina microarray.
We thank H. Fukuda and Y. Sugisawa for processing raw microarray data; S. Yonehara for providing c-Jun and A-Fos plasmids; G. Breton, K. Hitomi, T. Oyama, T. Muranaka and Y. Kondo for advice; T. Koto, K. Katayama and B. Y. Chow for technical assistance; J. A. Hejna and T. R. Endo for English proofreading.
Samples were lysed in SuperAmp Lysis Buffer and sent for processing and microarray analysis.
We thank Donatella Biancolini (Genopolis, Milan, Italy) for processing the microarray experiments of infected Mφs and DCs, and Ottavio Beretta and Federico Vitulli (Genopolis, Milan, Italy) for bioinformatics support.
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