Median time to capture a single image was 13 s (5 24), while the whole examination took 182 s (141 238) excluding time for probe change.
In our study, learners obtained a single image in less than 30 s in 97% of the cases and, excluding time for probe change, achieved the whole ultrasound protocol in less than 4 min.
The results reported here show the JR1 out-performs these dyes by a factor of 5 10, and indicate that porphyrin-based dyes may be useful for probing fast changes in membrane potential.
Given these limitations of DTI, alternative diffusion MRI models measuring non-Gaussian water diffusion, such as diffusion kurtosis (Jensen et al. 2005; Veraart et al. 2011b), may be more sensitive for probing microstructural changes in regions with complex tissue architectures (Wu and Cheung 2010; Blockx et al. 2012).
Our Drosophila model allows for probing the metabolic changes at multiple points in development, and consequently presents an opportunity for the discovery of mechanisms that alter early developmental metabolic pathways, providing a foundation for future focused studies in mammalian systems.
However, the incubation temperature for probes was changed to 60°C to reduce the background.
The same U251 tumor RNA pools (comparisons B and C, above) were also analyzed on Agilent Whole Human Genome oligonucleotide microarrays (4 × 44 K platform, version 2; platform GPL10332, array design #026652, containing 34,127 unique probes) to probe for changes in expression of (human) tumor RNAs.
HP0593 MTase has four tryptophans at positions 40, 163, 268 and 304, which provide a basis for probing ligand-induced conformational changes.
Taken together, cells expressing CXCR4CFP, CCR5YFP, CCR5STAYFP and CCR5Δ4YFP provide a system for probing extracellular ligand-induced changes in the dynamics distribution of these receptors on plasma membrane of live cells.
Frey and Wang have developed hydrogel compositions for 2D culture in which modulus can be decreased with UV irradiation and degradation, providing a method for probing cellular response to changes in substrate rigidity (Fig. 18).
