Based on the results of specificity analyses in the literature [ 32] and BLAST [ 33] searches focusing on the bp1241 bp132626 region of the IS1111 transposon which failed to identify organisms other than C. burnetii with the specific targeted sequence, the specificity of the PCR was defined as 100%% for purposes of prevalence analysis.
The RR itself and its lower and upper 95% confidence limits (LCL and UCL) are always recorded, with the odds ratio chosen if available for a prevalence analysis and the relative risk (or hazard ratio if provided) for an incidence analysis.
Population data for the practice, aggregated along the same age bands, was also uploaded for AF prevalence analysis.
Transfers from GP or non-governmental programmes (n = 8) were included for TB prevalence analysis but removed before incidence and survival analysis because of unknown previous ART regimens.
For the prevalence analysis, participants who had a stroke at baseline (963, 7.4%) or for whom baseline information about stroke was missing (138, 1.1%) were excluded, leaving 11 903 participants.
Overall and monthly household income-specific prevalence were adjusted for age, sex, smoking status, occupational position, marital status, dependent children at home (age <15 years), education, Swiss citizenship, complementary health insurance, receiving health insurance premium subsidy, and monthly household income (only for overall prevalence analysis).
For the symptom prevalence analysis, the annual mean NO2 concentration in the year immediately prior to the survey year was assigned as the exposure.
For the disease prevalence analysis, a 3-year average annual NO2 exposure was assigned (over the year of the survey and 2 years prior) to correspond to the 3-year time period in the survey outcome questions.
Finally, the questionnaire did not include questions regarding sexual behaviour, precluding HSV prevalence analysis for these variables.
After being stratified by gender, the association was significant for parental divorce (OR 3.76, 95% CI 2.34 to 6.03) in the prevalence analysis and for being raised in a relative's house (OR 1.89, 95% CI 1.21 to 2.94) in the incidence analysis only among women.
