As improved tools make transgenesis more and more efficient, the stage has been set for precise modification of the zebrafish genome such as are done in other model organisms.
The most immediate application, aside from generating loss-of-function mutations, will be to use donor DNA for engineering precise modifications, including transgene insertion.
Such precise modifications are desired for targeted genome engineering.
Additionally, the optimal assignment on molecular graphs has the advantage to investigate and interpret the mappings, allowing precise modifications of internal parameters of the similarity measure for specific targets.
However, the application of techniques involving HR for precise genome modification is still not routine.
Tailor-made nucleases for precise genome modification, such as zinc finger or TALE nucleases, currently represent the state-of-the-art for genome editing.
Understanding water stress tolerance is essential for precise trait modification.
Precise modification of plant genomes provides powerful tools for understanding gene function.
Precise modification of oxide surfaces by surface engineering is known to exhibit better photoactivity.
Therefore, technologies that facilitate the precise modification of cellular genomes have diverse and significant implications in many facets of research and are essential for translating the products of the Genomic Revolution into tangible benefits for medicine and biotechnology.
