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The renal arteries were dissected out under ×4 magnification on ice and stored at −80 °C for posterior analyses.
and store for posterior analyses.
From this filtering, 7970 different nrESS were considered for posterior analyses.
Only loci with sequence depth above the estimated x* value were retained for posterior analyses.
Thus, no hierarchical population structure was detected and the two original groups (fiber and linseed) were adopted for posterior analyses.
Those variables showing VIP values greater than 0.83 and which VT2 was at least 40%, were retained for posterior analyses.
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Tissue and excess mitochondrial samples were stored at −80 °C for the posterior analyses.
Therefore, we recognized Tasmania, Antarctica, New Zealand and the Macquarie Ridge as four distinct populations for all posterior analyses.
Fragment sizes were estimated using GeneScan ROX-500 and MapMarker® 1000 (BioVentures Inc., Murfreesboro, TN) internal size standards, and the genotypic data matrix generated was used for all posterior analyses.
For the MCMC posterior analyses, the length of chain was 10 000 000.
We used a maximum clade credibility (MCC) tree and a sample of high posterior probability trees from the BEAST results for later analyses.
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