First using the "Right Dihedron Method", a graphical method for the determination of possible orientations of σ1 and σ3, which are independent of the choice of the nodal planes (Angelier and Mechler, 1977).
Recent work has documented orientation-dependent fracture and fatigue properties of as-deposited Ti 6Al 4V manufactured by electron beam melting, in addition to providing a modified ASTM nomenclature to begin the discussion of possible orientations to consider for mechanical characterization.
For that, we calculated gradient-sign maps, not just for one, but for all possible orientations between 0 and 179°, in 1°-steps.
For each edge A – B, NEO generates edge orienting scores for both possible orientations: A → B and B → A. If an erroneous edge exists between two traits, then it is meaningless to orient it.
This implies that crystallization selected a specific interdomain orientation from an ensemble of possible orientations.
As shown in Figure 2, over 50% of the designed sense and antisense probes did not produce a single positive signal when hybridized to the pooled cRNA sample, which is in line with our expectation, given one possible orientation for each target EST.
This was done for both possible orientations as the database contains sequences derived from 5' as well as 3' cDNA ends.
For compound 3, the disorder was modeled by running the Hirshfeld surface analysis for both possible orientations of the propyl esters.
Since four different orientations of l-alanine molecules are possible on {011} and {120} interfaces, the calculations on each surface are repeated for all possible orientations as shown in Figure 5.
PCR mutant screening was carried out with Eppendorf Taq DNA polymerase (Eppendorf) with pooled transposon mutants as templates, IS 6100-specific primers IS6100x and IS6100y for both possible orientations of the transposon and a position-specific search primer gs_trpP (Table 3).
