Sentence examples for for plasmid preparations from inspiring English sources

We thank C.F. Burant and J.D. Lin for helpful discussions, J. Washburn and J.W. MacDonald for performing the microarray analysis, G. Bommer for assistance with GSEA, T. Lanigan for technical support of virus production, and S. Borofsky for plasmid preparations.

Escherichia coli strain DH5α was used for plasmid preparations.

All chemicals for bacteria culturing and the GenElute™ plasmid extraction kit for plasmid preparations were sourced from Sigma Aldrich (UK).

Second, pBluescriptKS phagemid from the Lambda ZAP vectors was mass excised from both libraries and individual colonies grown for 24 h in a volume of 3 ml for plasmid preparations.

E. coli strains Top 10F′ and BL21 (DE3) were used as hosts for plasmid preparation and recombinant protein expression, respectively.

E. coli DH5α (Takara Bio, Otsu, Japan) and E. coli BL21 (DE3) (Takara Bio) were used for plasmid preparation and sequencing and for expression and purification of recombinant CgPAD, respectively.

We thank Toshie Sakuma for plasmid preparation.

We now describe a fast, efficient and economic in-house protocol for plasmid preparation using glass syringe filters.

Bacterial colonies were pooled and used for plasmid preparation.

The E. coli DH5α strain was used for plasmid preparation and all restriction-based cloning.

The cells were analyzed by SDS-PAGE atd athehe same time used for plasmid preparation.