For plasma membrane labeling, cells were incubated with CTB Alexa 594 or CTB Alexa 647 (10 µg/ml) for 30 min at 4 °C and then washed with fresh media.
In all experiments, semen was assessed for plasma membrane integrity and for percentage of motile and progressive sperm by CASA.
Thiery J, Keefe D, Saffarian S, Martinvalet D, Walch M, Boucrot E, Kirchhausen T, Lieberman J. Perforin activates clathrin- and dynamin-dependent endocytosis, which is required for plasma membrane repair and delivery of granzyme B for granzyme-mediated apoptosis.
Thawed sperm were evaluated for plasma membrane integrity and quality, motility, acrosome integrity and, after artificial insemination, for egg fertility and hatchability.
Potassium channels in the inner mitochondrial membrane are modulated by inhibitors and activators (potassium channel openers) previously described for plasma membrane potassium channels.
Cholesterol is an essential structural component for plasma membrane both in brain and peripheral tissue.
Hence, the method can be applied to wild-type and diseased cell-type (for example cancer cell) for plasma membrane and endosomal compartmental isolation.
We particularly show that phospholipid functionalized SPMNPs and dimercaptosuccinic acid (DMSA) functionalized SPMNPs can be used for plasma membrane and endosomal enrichment respectively.
In silico analysis revealed two components of S. cumini methanolic extract to be active against four enzymes (PDB ID 1W5D, 4OX3, 3MFD and 5E2F) which are crucial for plasma membrane synthesis in B. subtilis.
For plasma membrane marker, sodium potassium ATPase was used.
Alcohols can be used as a model for plasma membrane [41].
