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We thank Jianling Wang and Lu Tian for assistance printing and hybridizing microarrays, Ting-Li Lin for statistical support in the analysis of real time RT-PCR data, The University of Wisconsin Biotron Facility personnel for assistance with setting up controlled growth conditions for plant experiments, and two anonymous reviewers for providing useful suggestions for improving the manuscript.
We thank Oliver Castillo, J. L. Zitlalpopoca, and Hadau Sánchez for plant experiments and greenhouse support, and María del Carmen Vargas for technical assistance.
Additionally, the contemporary access to the ISS, along with new orbital habitats and hardware for plant experiments, will enable researchers to design a new generation of replicated experiments with which to explore these phenomena.
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Finally, we argue that the same approach is useable for an optimal design of plant experiments.
The objective function for designing plant experiments includes the gain in profit after reducing the offset and variability by experimentation, and loss in profit during experimentation.
The objective function for designing plant experiments includes the gain in profit by reducing the offset and variability after the experiments and loss in profit before and during experimentation.
The experimental design was a completely randomised block with three biological replications (treatment of three individual barley ears per treatment group) for both plant experiments.
For the stationary plant experiments, we constructed a less rigid plant out of strips of black plastic trash bag material.
This paper presents a new criterion to design plant experiments for model-based real-time optimization (RTO) systems.
This paper presents a new profit-based criterion to design plant experiments for model-based real-time optimization (RTO) systems.
Culture media and growth conditions for R. etli, and plant experiments were accomplished as previously described in reference [ 45].
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