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Such a classification will be useful for physical mapping and eventual sequencing as high repetitive clones can be avoided.
As HGP sequencing efforts accelerated, concerns arose that only four genomes, a couple of which belonged to known laboratory personnel, were being used for physical mapping and sequencing (Marshall 1996b).
This ability is important for physical mapping and sequencing projects especially for large genomes where numerous blocks of highly repetitive sequences cause gaps in the maps due to the absence of unique markers.
In this study, we evaluated the potential of WGP for physical mapping and sequencing of complex genomes like wheat.
Libraries with large genomic DNA inserts are essential for physical mapping and positional cloning, particularly for higher eukaryotes [ 8].
Although MjBL2 is not suitable for physical mapping and genome sequencing because it was constructed from 13 shrimps, MjBL2 is useful as the first step for characterizing the kuruma shrimp genome.
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Here, we demonstrate the use of fluorescence in situ hybridization with bacterial artificial chromosome probes as a powerful tool for physical mapping of genes and comparative genome analysis in Lepidoptera, which have numerous and morphologically uniform holokinetic chromosomes.
The flexibility of the BAP mapping allows conversion of BAC landing data into BAC linkage contigs, utilizable for ongoing physical mapping and sequencing projects, especially for organisms with a large genome size.
The fully sequenced genomes of model species provide fundamental resources for translational genomics, and can potentially act as scaffolds for both physical mapping and next-generation whole genome sequencing of closely related species.
A critical tool for genomic studies in fish species is the availability of deep-coverage large-insert genomic libraries, such as BAC libraries that can be used for physical mapping, integration of linkage and physical maps, positional cloning, comparative genomic studies and genome sequencing [ 13].
A particularly promising strategy for simultaneous physical mapping and sequencing of large eukaryotic genomes involves sequencing pools of sheared, individually "bar coded" BAC clones.
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