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Comparison of the relaxation characteristics of the interfibrillar/interspherulitic phase with those of pure PEI indicated a much broader spectrum of local relaxation environments for PEI in the blends, consistent with PEI segregation across a wide range of size scales.
After PEI modification, the functionalized nanocomposite displayed a peak at 230 nm that was typical for PEI.
For PEI, the number of biotin groups present on the complex did not affect transgene expression.
Results confirm the decrease of solvent power for PEI in the series: NMP > DMAc > DMF.
For Pei, that meant looking beyond the American geography that had dominated his imagination since the 1950s: his postretirement projects have taken him to Japan, Spain, Greece, England, Germany, Luxembourg, China, and Macao.
Surfaces densities ranging from 0.4 to 2.6 μg DNA/cm2 were obtained for PL, and from 0.7 to 1.0 μg DNA/cm2 for PEI.
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Future studies could examine adsorption and desorption properties for PEI-CNT fibers and compare them to CNT yarns, but these experiments suggest PEI-CNT fiber microelectrodes could be useful for detection of dopamine with fast repetition rates.
Our optimized settings for PEI-mediated TGE in HEK and CHO cells evaluated on several proteins are generally applicable to recombinant antibodies and proteins.
The characteristic ratios of 4.9±0.1 for PEO-4 and 3.1±0.1 for PEI-4 are consistent with experiment and with theoretical predictions.
Here, we give some insight into strategies developed for PEI-based non-viral vectors to overcome intracellular obstacles, including the improvement of methods for polyplex preparation and the incorporation of endosomolytic agents or nuclear localization signals.
The amount of grafted PEI estimated by thermogravimetric analysis was 5.08% (w/w) and 5.28% (w/w) for PEI-NH-SWNTs and PEI-NH-MWNTs, respectively.
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