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Percent normalized RMS errors for peak height, F2 peak density, and propagation factora.
The precisions (RSDs) of 100 consecutive injections are 2.43%, 3.86%, and 4.25% for peak height, peak area and migration time, respectively.
A useful notation is I for peak height, so here, I G) is the height of the G peak, as shown, and the I(G /I 2D) peak ratios are 0.6 and 0.7 for 2.5 and 5 min, respectively.
The precision of the method was evaluated in terms of repeatability and reproducibility obtaining values lower than 1.1% for drift time and 12.0% for peak height when the membrane was used coupled to the UV-IMS for all target analytes in the humidity range of 10 75% RH.
However, despite an increase in peak dimensions, the majority of peaks still displayed similar properties to the mock-hybridized region, as observed in cumulative frequency plots for peak height (Figure 5E) and diameter (Figure 5F).
‡Missing data for peak height (n=5) and gradient (n=4).
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The target CpGs were evaluated by converting the resulting pyrograms into numerical values for peak heights, and calculating the average of all CpG sites analyzed at a given gene promoter (Figure S2).
Since both LOD and LOQ refer to signal heights, calibration curves for peak heights had to be calculated.
Linearity for peak heights in the low concentration range (0.5 4.5 μg/ml) was confirmed by Mandel's fitting test (data not shown).
For reasons of completeness, LOQ was calculated according to the ICH guideline dividing the tenfold standard deviation of the signal fluctuation of the noise by the slope of the calibration curve for peak heights.
Target CpG sites were evaluated using the instrument software (PSQ96MA 2.1, Qiagen, Valencia, CA), which converts programs to numerical values for peak heights and calculates the proportion of methylation at each base as a C/T ratio.
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