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The establishment of rapid cloning, expression and protein detection procedures has therefore become a major field of interest for the design of high-throughput methods for parallel expression of proteins in multiple expression systems.
The CD133 positive subpopulations within the xenograft panel were further analysed for parallel expression of CD44 and CD24 or CDCP1.
The method is applicable to several cell-types and can be scaled for parallel expression measurements of hundreds of gene promoters.
We developed a method for parallel expression and purification of recombinant proteins with a hexahistidine tag (His-tag) or glutathione S-transferase (GST -tag from bacterial expression systems.
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"So it was important to me to capture the cadence of the original language, to have a kind of parallel expression for that in English, without it sounding odd".
Investigation of the chromosomal location of these miRNAs confirmed a reasonable explanation for the parallel expression - they are encoded in the same transcript [ 9, 18].
These considerations motivated the proposal, in the 2005 version of this article, that mou referred very generally to taking any two parallel expressions, "proceeding" to assert a further expression on the basis of one of them, and then using that as grounds for asserting a parallel expression on the basis of the other.
This feature could be an enhancer or an LCR common to both genes and responsible for their observed parallel expression profiles.
The cGRNB enables two major network-building modules, one for MPGE (miRNA-perturbed gene expression) dataset and another for parallel miRNA/mRNA expression datasets.
The cGRNB enables two major network-building modules, one for MPGE (miRNA-perturbed gene expression) datasets and the other for parallel miRNA/mRNA expression datasets.
Specifically, we performed parallel expression analyses for MC1R mRNAs in the two lizard population (n = 5 males and 5 females for each population, see Additional files for full details).
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