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Since both the study area and Okinawa are in the Kuroshio region, temporal changes in ΔR will affect the calibration of 14C ages used to provide calendar dates for our specimens.
Age information is not available for our specimens, so we use size as ontogenetic scale (i.e. ontogenetic allometry).
However, this is not true for our specimens from 4000 m, which have a pure C3 diet.
For our specimens the density of GCs ranged between 11400 (at the central retina) to 4000 cell/mm (peripheral retina), with an average of 8100 cells/mm.
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This required titling angle exceeds the tilting limit of ±30° for our specimen holder.
We deeply thank Elisabeth Errazuriz and Simone Peyrol (Centre Commun d'Imagerie de Laennec) for processing our specimens for EM analysis, Sylvie Fiorini and Avril Trejdosiewicz for their technical assistance, and Cathy Berthet for her valuable secretarial help.
We are grateful to Ray Berkelmans for confirming the identity of our specimen of Acropora microphthalma based on the taxonomy of [27].
Now we are waiting for our frozen specimens to be sent to the States so that the rest of the lab work can be done, including collecting additional data on the nutritional quality of the macroalgal species for my master's thesis.
Also, we used as controls hippocampi from necropsy with longer delays before fixation than those for our surgical specimens.
While it is difficult to translate the findings of these studies to the work performed here because the amount of micromotion was not controlled, it is interesting to note that most of the micromotions at the interface for our retrieval specimens was less than 100 μm, with about half having cement-bone micromotions of less than 10 μm.
Although our sample set was selected for certain mutations, our specimens contained variants that represent the full spectrum of mutations that are identified in NSCLC and CRC specimens.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com