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Moreover, at least one commonly used reference gene, β-actin, was differentially regulated between NS and OS in the microarray study (Table S2, ProbeName UF_Sgi_AF_101275), making it a poor candidate for our internal reference in real-time RT-PCR.
We designed five primer pairs for qPCR amplification, and two primer pairs for our internal reference (supplementary table S5, Supplementary Material online).
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"The interior is more refined, but our internal reference for the exterior is 'badass styling,'" George said.
Retention times of the different polyamines were as follows: 1.8 min for DP, 2.2 min for PU, 3.35 min for DH (diaminoheptane, internal reference), 5.46 min for SD and 6.63 min for SM.
For example, ℙ(C1) > 0.7 for internal reference panels of n1 ≥ 400 haplotypes even when considering an extremely large external panel of n2 = 10,000 haplotypes (light blue curve).
We conclude that empirical modeling based on an abiotic stressor gradient can provide an alternative approach for deriving internal reference expectations for great rivers with few, if any, minimally disturbed sites.
Primers and probe for the internal reference gene, ACTB (GenBank accession no.
The TERT locus was used for the internal reference copy number.
We used type II collagen gene (COL2A) for an internal reference gene by amplifying the non-CpG sequences.
Several repetitions were used to establish the efficiency of the standard curve for the internal reference gene.
Both sets of criteria are presented: the WHO classification for comparison across published studies and the Chinese classification for an internal reference.
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