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To analyze the lipid synthesis and BCR-directed DAG production in wild type primary B cells, we systematically assessed the numbers of primary B cells and the amount of radioactivity required for optimum labeling.
Fluorescence measurements justified the choice of 2 mol % for optimum labeling, because 1), this is near the limit of the linear relationship between the fluorescence monomer intensity and the Py-met-chol concentration (Fig. 5); 2), it generates the largest amplitude of I1/ I3 variations (Figs. 5 b and 6 a ); and 3), it allows an accurate quantification of the excimer (Fig. 5).
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Earlier work showed that it is possible to label DATA chelators over the pH range 4 7, however for the purposes of this work it was decided that only optimum labelling conditions would be used in line with the desire to develop a room temperature kit-type labelling protocol (Waldron et al. 2013; Seemann et al. 2015a).
Read up on germination ranges for optimum emergence.
The shape will also allow for "optimum solar and wind power generation", apparently.
A result is that many older people frequently drink fewer liquids than needed for optimum health.
Fruit trees require "chilling hours", or time in cold, wintry environments, for optimum production.
For optimum viewing, "Don Quixote" should be seen on the largest possible screen.
And Mr. Walker comes to your listening room to tweak every setting for optimum performance.
Watch and read for optimum review experience.
The concentration of AQMS label has also been optimised in the measuring electrolyte between 0.1 and 5.0 mM, and the concentration of AQMS at 1 mM was found to be sufficient for optimum DNA intercalation reaction (Fig. 4b).
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